Aminoferrocene‐Based Anticancer Prodrugs Labelled with Cyanine Dyes for in vivo Imaging

N-alkylaminoferrocene-based (NAAF) prodrugs are activated in the presence of reactive oxygen species (ROS), based on which these target cancer cells (high ROS) and do not affect normal (low ROS). To gain some insights into their mode action vivo, we have investigated biodistribution 18F-labelled NAAF tumor-bearing mice by positron emission tomography (PET). Due to short half-life 18F, experimental time frame was restricted 60 min. extend observation window, a more stable marker is required. In this paper, report conjugates with two cyanine dyes Cy5 Cy7 including details synthesis, characterization basic properties cell free settings cellular uptake representative human cells. Finally, conjugate subjected vivo fluorescence imaging studies determine prodrug over 24 h.